The occurrence of nuclear migration under thiol treatment effective in inhibiting neurulation.
نویسنده
چکیده
Early chick embryos were explanted on culture media containing mercaptoethanol and dithiothreitol in concentrations reaching 10~ M and 10~ M respectively. Nuclear migration was found to occur normally even under such thiol concentration, which is more than required to inhibit neurulation. Electron miscroscopy revealed that microtubules were not affected in their number and morphology. However, nuclear migration was blocked with 2-5X10~M colchicine; such treated embryos showed a loss of their microtubules. It is concluded that the two thiols do not act primarily on microtubules when they cause inhibition of neurulation.
منابع مشابه
Inhibition of nuclear migration in the absence of microtubules in the chick embryo.
Tnterkinetic nuclear migration was studied in cells of the forming neural tube of cultured chick embryos aged from 1 to 7 pairs of somites. In specimens that were first exposed to 2°C for 3 h, then treated with 4 x 10~ M monoiodoacetamide and finally transferred to new media to be reincubated for 1-2-5 h, it was found that microtubules were absent and nuclear movements blocked. These findings p...
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ورودعنوان ژورنال:
- Journal of embryology and experimental morphology
دوره 27 3 شماره
صفحات -
تاریخ انتشار 1972